Clinical and Prognostic Value of Endothelial NO-Synthetase Gene Polymorphism in Patients with Acute Coronary Syndromes

A.N. Parkhomenko, Ya.M. Lutay, O.I. Irkin, S.N. Kozhukhov, А.A. Skarzhevsky, V.Ye. Dosenko, A.A. Moybenko


Nitric oxide (NO) is produced by endothelial nitric oxide synthetase (eNOS) and plays important role in normal vascular homeostasis. In the eNOS gene various polymorphisms have been identified. The -786T>C polymorphism in the gene promoter has been demonstrated to modulate the eNOS gene expression and so the synthesis of NO.
325 patients with ACS and 83 healthy controls were investigated. The eNOS polymorphism has been analyzed by polymerase chain reaction and restriction fragment length polymorphism. In Ukrainians the C/C genotype in the eNOS gene promoter was more common than in Japanese and less common than in west Europeans, north Americans and Australians. A significant difference in genotype distribution was observed between patients and controls. Pathological C/C genotype was significantly more often determined in ACS patients, especially in men younger than 55 years old.
-786CC genotype was associated with increase in arterial stiffness and lower flow-mediated brachial artery dilatation. -786TT variant of the eNOS gene promoter in investigated patients was associated with better efficacy of reperfusion therapy. Patients with -786TT genotype had fewer complications during the period of hospitalization.


gene polymorphism; endothelial NO-synthetase; endothelial dysfunction; pulse ware velocity; acute coronary syndrome; thrombolytic therapy


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