DOI: https://doi.org/10.22141/2224-0586.1.80.2017.94469

Factors influencing the platelet concentration and functional properties in plasma rich in growth factors (PRGF Endoret)

V.A. Rybak, L.V. Natrus, A.V. Kopchak, T.O. Pavlychuk, P.A. Chernovol

Abstract


The aim of the study was to examine the platelet concentration in plasma rich in growth factors (PRGF Endoret) obtained by standard protocol and its functional properties depending on the patient’s individual hematological features. The study examined 30 patients with post-traumatic and post-operative defects of the upper and lower jaw. Before reconstructive surgery the clinical and radiological examination, blood test, coagulation and induced platelets aggregation were examined. During the reconstructive surgery two plasma fractions were produced by the PRGF Endoret protocol for all patients. The platelets and other blood cells concentrations, as well as the fibrin membrane morphology were evaluated for each fraction. The average ratio between the concentration of platelet in fraction rich in growth factors (F2) and native blood (concentration index) was 1.48, the platelets content in F1 fraction (plasma poor in platelets) was 1.3 less than in blood. The main factors that influence the platelet concentration in plasma were the initial number of platelets in blood, hematocrit and fibrinogen concentration. The ratio of platelets level in fractions depended only on hematocrit. The study of correlations between the functional activity of platelets in F1 and F2 fractions revealed the inverse correlation between platelets aggregation activity and their content in F2 fraction. This explains the absence of statistically significant influence of platelet amount in PRGF on clinical efficiency of surgical procedures. In non-standard cases, when hypoactive platelet characteristics were associated with their increased amount in blood it was possible to use PRGF method without division on fractions. In cases, when small number of platelets in blood was associated with their hypoactivity, a larger (double) volume of PRGF (F2 fraction) was recommended for use to provide the better therapeutic effect.

Keywords


fibrin; growth factors; platelet; platelet-rich plasma; PRGF

References


C.E. Sommeling, A. Heyneman, H Hoeksema, J Verbelen, F.B. Stillaert, S. Monstrey. Theuseof platelet-rich plasmainplasticsurgery: a systematicreview. JournalofPlastic, Reconstructive&AestheticSurgery. 2013;66(3):301–311.doi: 10.1016/j.bjps.2012.11.009

A. Suchetha, P. Lakshmi, D.Bhat, D.B. Mundinamane, K.V. Soorya, G. Ashit. Plateletconcentrationinplateletconcentratesandperiodontal regeneration-unscrambling theambiguit. BharwaniContempClinDent. 2015;6(4):510–516.

C.W. Hsu, K. Yuan, C.C. TsengHsu C.W. Thenegativeeffectof platelet-rich plasmaonthegrowthofhumancellsisassociatedwithsecreted thrombospondin-1.OralSurgOralMedOralPatholOralRadiolEndod. 2009;107:185–92.

I.Giusti, S. D'Ascenzo, A. Mancò Et al. Plateletconcentrationin platelet-rich plasmaaffectstenocytebehaviorinvitro.BiomedResInt. 2014.

G. Weibrich, W.K. Kleis, W.E. Hitzler, G. Hafner. Comparisonoftheplateletconcentratecollectionsystemwiththe plasma-rich-in-growth-factors kittoproduce platelet-rich plasma: a technicalreport. J OralMaxillofacImplants. 2005;20(1):118-123.

Inchingolo F, Tatullo M, Marrelli M et al.Regenerativesurgeryperformedwith platelet-rich plasmausedinsinusliftelevationbeforedentalimplantsurgery: anusefulaidinhealingandregenerationofbonetissue. Eur Rev Med Pharmacol Sci. 2012;16(9):1222-6.

T.E. Foster, B.L. Puskas, B.R. Mandelbaum, M.B. Gerhardt, S.A. Rodeo. Platelet-rich plasma: from basic science to clinical applications.American Journal of Sports Medicine.2009;37(11):2259–2272.doi: 10.1177/0363546509349921

Giraldo C.E. Effects of sodium citrate and acid citrate dextrose solutions on cell counts and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel. BMC Vet Res. 2015;11:60.doi: 10.1186/s12917-015-0370-4

Marx R.E. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg. 2004;62:489–496. doi: 10.1016/j.joms.2003.12.003

10. T.M. McCarrel, T Minas, L.A. Fortier. Optimization of leukocyte concentration in platelet-rich plasma for the treatment of tendinopathy. J Bone Joint Surg Am. 2012;94:143.doi: 10.2106/JBJS.L.00019.

Marx R.E. Platelet-rich plasma (PRP): what is PRP and what is not PRP?Implant Dent. 2001;10:225–228.doi: 10.1097/00008505-200110000-00002

G. Weibrich, T. Hansen, W. Kleis, R. Buch, W.E. Hitzler. Effect of platelet concentration in platelet-rich plasma on peri-implant bone regeneration J Bone. 2004;34:665–671.

E. Lopez-Vidriero, K.A. Goulding, D.A. Simon, D.H. Johnson, M. Sanchez. Poor standardization in platelet-rich therapies hampers advancement. Arthroscopy: The Journal of Arthroscopic and Related Surgery.2010;26(6):724–725. doi: 10.1016/j.arthro.2010.04.002.

E.Anitua, R. Prado, M. Troya et al.Implementation of more physiological plasma rich in growth factor (PRGF) protocol: Anticoagulant removal and reduction in activator concentration. J Platelets. 2016;27(5):459-466.

E. Anitua, R. Prado, G. Orive. Endogenous morphogens and fibrin bioscaffolds for stem cell therapeutics.TrendsinBiotechnology. 2013;31(6):364–374.

Armitage P., Berry G., Matthews J.N.S. Statistical methods in medical research. -2002. 4th edn. Blackwell Science Ltd, London

BlendM., Introduction to medical statistics.-2000. 3th end.Oxford: Oxford

OvcharovA.O. Actual problems of the scientific research: Methodology, Economics, Statistics. Collections of articles

M Kawasumi, H Kitoh, K.A. Siwicka, N Ishiguro. The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone.Bone Joint Surg Br. 2008;90:966–972. doi: 10.1302/0301-620X.90B7.20235.

M.G. De Andrade, C.J. FreitasBrandão, C.N. Sá, T.C. de Bittencourt, M Sadigursky.Evaluation of factors that can modify platelet-rich plasma properties.Oral Surg Oral Med Oral Pathol Oral RadiolEndod.2008;105:5–12. doi: 10.1016/j.tripleo.2007.07.032

G Weibrich, W.K. Kleis, M. Kunz-Kostomanolakis, A.H. Loos, W. Wagner.Correlation of platelet concentration in platelet-rich plasma to the extraction method, age, sex, and platelet count of the donor.J OralMaxillofac Implants. 2001;16(5):693-699.

J.D. Bessman, L.J. Williams, P.R. Gilmer. Mean platelet volume. The inverse relation of platelet size and count in normal subjects, and an artifact of other particles. J Clin Pathol.1981;76(3):289-293.

Sundman E.A.et al.Growth fsctor and catabolic cytocine concentrations are influenced by the cellular composition of platelet-rich plasma. J Sports Med.2011;39(10): 2135-40.

Anitua E. The use of plasma-rich growth factors (PRGF) in oral surgery.J PractProcedAesthet Dent. 2001;5(6):487-493.

McCarrel T.M. Optimization of leukocyte concentration in platelet-rich plasma for the treatment of tendinopathy. J Bone Joint Surg Am. 2012;94:143. doi: 10.2106/JBJS.L.00019.

D.T. Graves, D.L. Cochran. Biologically active mediators: platelet-derived growth factor, monocyte chemoattractant protein-1 and transforming growth factor-beta. CurrOpin Dent.1991;1:809–815.

R.J.F.C. doAmaral, N.P. da Silva, N.F. Haddad, L.S. Lopes, F.D. Ferreira, R.B. Filho, P.A. Cappelletti, W. de Mello, E. Cordeiro-Spinetti, A.Balduino. Platelet-rich plasma obtained with different anticoagulants and their effect on platelet numbers and mesenchymal stromal cells behavior in vitro. Stem Cells Int. 2016.doi: 10.1155/2016/7414036

M. Pakyari, A. Farrokhi, M.K. Maharlooei, A. Ghahary. Critical role of transforming growth factor beta in different phases of wound healing. Advances in Wound Care.2013;(5):215–224. doi: 10.1089/wound.2012.0406

D.I.R. Holmes, I. Zachary. The vascular endothelial growth factor (VEGF) family: angiogenicfactors in health and disease. GenomeBiology.2005;2:209. doi: 10.1186/gb-2005-6-2-209.

MylodarovM.Yu.Effect of platelet and aggregation process to the erythrocyte interaction Yaroslavl Pedagogical Gazette.- 2013.

KejanD.TheuseofplateletrichplasmaforsurgicaltreatmentofradicularcystsofthejawinmaterialInternational scientificconference «Modern dentistry and maxillo-facial surgery». Kiev. 2013.

G.F. Biloklytska, O.V. Kopchak. Theuseofplatelet-richplasma (PRP) fortreatmentofgeneralizedperiodontitis. Stomatologiawspotczesna.2014;21(3):8-17.

Andrade MG, de FreitasBrandão CJ, Sá CN, de Bittencourt TC, Sadigursky M. Evaluation of factors that can modify platelet-rich plasma properties. Oral Surg Oral Med Oral Pathol Oral RadiolEndod. 2008;105(1):5-12.doi: 10.1016/j.tripleo.2007.07.032.




Copyright (c) 2017 EMERGENCY MEDICINE

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

© Publishing House Zaslavsky, 1997-2018

 

   Seo анализ сайта