Biochemical blood parameters of rats with infravesical obstruction with the introduction of biologically active compositions containing neurotrophic factors

Authors

  • V.Yu. Globa Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
  • Ya.Yu. Samburg Kharkiv Medical Academy of Postgraduate Education, Kharkiv, Ukraine
  • G.A. Bozhok Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
  • E.I. Legach Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

DOI:

https://doi.org/10.22141/2224-0586.16.4.2020.207940

Keywords:

obstructive uropathy, spinal ganglion, satellite cells, cryoextract, cryopreservation, biochemical parameters of the blood

Abstract

Infravesical obstruction occurs in many urological diseases. At the same time, obstructive uropathy develops, which can lead to renal failure with subsequent disability and death of patients. Currently, treatment methods alternative to chronic hemodialysis and kidney transplantation that contribute to the structural and functional restoration of the kidneys have not been found. Biochemical parameters of the blood (levels of total protein, urea, and creatinine) were investigated in female rats with infravesical obstruction after the administration of biologically active compositions, which contain neurotrophic factors. An experimental model was obtained by applying a ligature to the distal urinary tract. Spinal ganglia cryoextract, conditioned media from native and cryopreserved cultures of satellite cells, basic culture medium, and сortexin were used as biologically active compositions. Biologically active compositions were administered to animals intraperitoneally for 10 days. The indicators of protein and nitrogen metabolism were determined. The use of all biologically active compositions did not increase blood urea levels. At the same time, spinal ganglia cryoextract and conditioned media from native and cryopreserved cultures of satellite cells increased the levels of total protein by 51.5, 13.3 and 29.9 %, respectively, compared with the indices of control animals. Only rats with infravesical obstruction, who were injected with conditioned medium from the culture of cryopreserved satellite cells, did not show an increase in blood creatinine levels, the indices of which were similar to the control ones. The introduction of biologically active compositions containing neurotrophic factors does not lead to nephrotoxic effects due to the lack of influence on blood urea levels. Spinal ganglia cryoextract and conditioned media from native and cryopreserved cultures of satellite cells have an effect on increasing the level of total blood protein in rats with infravesical obstruction. Moreover, the conditioned medium from the culture of cryopreserved satellite cells demonstrates the absence of influence on the main pathways of creatinine metabolism in obstructive uropathy due to infravesical obstruction. Therefore, all of the above effects make it possible to use biologically active compositions from the spinal ganglia in the presence of renal pathology.

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References

Zuhirman - Zamzami. Blood urea and creatinine levels in obstructive uropathy patients due to benign prostate hyperplasia after transurethral resection of the prostate. Int J Surg Med. 2019; 5(1): 18-22. doi:10.5455/ijsm.

Wilson DR. Pathophysiology of obstructive nephropathy. Kidney International [Internet]. 2015 Dec 18 [cited 2020 Jan 12]; 18(3): 281-92. Available from: https://doi.org/10.1038/ki.1980. 138.

Iqbal S, Raiz I, Faiz I. Bilateral hydroureteronephrosis with a hypertrophied, trabeculated urinary bladder. Malays J Med Sci. 2017; 24(2): 106-15. Available from: https://doi.org/ 10.21315/ mjms2017.24.2.14.

Chevalier RL. Pathogenesis of renal injury in obstructive uropathy. Curr Opin Pediatr. 2006; 18(2): 153-60. doi:10.1097/01.mop.0000193287.56528.a4.

Rayner H, Thomas M, Milford D. Understanding Kidney Diseases. Cham (Switzerland): Springer International Publishing; 2016. 300p.

Smith DR, Tanagho EA, McAninch JW, Lue TF. Smith and Tanagho's general urology. 18th ed. New York (USA): McGraw-Hill Medical; 2013. 758p.

Oka M, Sekiya S, Sakiyama R, Shimizu T, Nitta K. Hepatocyte growth factor–secreting mesothelial cell sheets suppress progressive fibrosis in a rat model of CKD. J Am Soc Nephrol. 2019; 30 (2): 261-76. doi: 10.1681/ASN.2018050556.

Chaabane W, Praddaude F, Buleon M, et al. Renal functional decline and glomerulotubular injury are arrested but not restored by release of unilateral ureteral obstruction (UUO). Am J Physiol Ren Physiol. 2013; 304 (4): 432-9. doi: 10.1152/ ajprenal.00425.2012.

Ruiz E, Ferraris J. 25 years of live related renal transplantation in children: the Buenos Aires experience. Indian J Urol [Internet]. 2007 [cited 2020 Feb 17]. 23(4): 443-51. Available from: http://www.indianjurol.com/text.asp?2007/23/4/443/36720.

Papazova DA, Oosterhuis NR, Gremmels H, van Koppen A, Joles JA, Verhaar MC. Cell-based therapies for experimental chronic kidney disease: a systematic review and meta-analysis. Disease Models & Mechanisms. 2015; 8(3), 281-93. doi:10.1242/dmm.017699.

Sugandhi N, Srinivas M, Agarwala S, et al. Effect of stem cells on renal recovery in rat model of partial unilateral upper ureteric obstruction. Pediatr Surg Int. 2014; 30(2): 233-8. doi: 10.1007/ s00383-013-3456-8.

Asanuma H, Vanderbrink BA, Campbell MT, et al. Arterially delivered mesenchymal stem cells prevent obstruction-induced renal fibrosis. J Surg Res. 2011; 168(1): 51-9. doi:10.1016/j.jss. 2010.06.022.

Tsui CC, Shankland SJ, Pierchala BA. Glial cell line–derived neurotrophic factor and its receptor ret is a novel ligand-receptor complex critical for survival response during podocyte injury. Journal of the American Society of Nephrology. 2006; 17(6), 1543-52. doi:10.1681/asn. 2005080835.

Verge VM, Gratto KA, Karchewski LA, Richardson PM. Neurotrophins and nerve injury in the adult. Philosophical Transactions of the Royal Society B: Biological Sciences. 1996; 351(1338): 423-30. doi:10.1098/rstb.1996.0038.

Patent 64381 A Ukraine, IPC7 A61K35/12. Galchenko SYe, Shkodovska NYu, Sandomirsky BP, Grischenko VI. [Method of obtaining of xenogeneic organ extracts]. Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine. №2003054649; Filed 22.05.2003; Publ. 16.02.2004. Bul. N2. (In Ukrainian).

Alі SG, Sidorenko ОS, Bozhok GА. [Influence of nutrient medium composition on the morphological characteristics of culture of dorsal root ganglion cells of neonatal piglets]. The Journal of V. N. Karazin Kharkiv National University. Series «Biology». 2018; 30 (30): 49-59. (In Ukrainian).

Zhang NZ, Ma L, Zhang JB, Chen J. Improved model for the establishment and evaluation of detrusor overactivity in female Wistar rats. Int Braz J Urol. 2014; 40 (3): 414-22. doi: 10.1590/ S1677-5538.IBJU.2014.03.17.

Shavlovskaya OA. [Clinical efficacy of neuropeptides in cerebrovascular pathology]. S.S. Korsakov Journal of Neurology and Psychiatry. 2016; 116 (8): 88-93. (In Russian). doi: 10.17116/jnevro20161168188-93.

Goryachkovskiy AM. Klinicheskaya biokhimiya v laboratornoy diagnostike [Clinical biochemistry in laboratory diagnostics]. Odessa: Ekologiya; 2005. 616p. (In Russian).

Hazel SJ, Gillespie CM, Moore RJ, Clark RG, Jureidini KF, Martin AA. Enhanced body growth in uremic rats treated with IGF-I and growth hormone in combination. Kidney International. 1994; 46(1): 58-68. doi: 10.1038/ki.1994.244.

Levin R, Chichester P, Levin S, Buttyan R. Role of angiogenesis in bladder response to partial outlet obstruction. Scand J Urol Nephrol Suppl. 2004; 38 (215): 37-47. doi: 10.1080/ 03008880410015156.

Barnett MW, Fisher CE, Perona-Wright G, Davies JA. Signalling by glial cell line-derived neurotrophic factor (GDNF) requires heparan sulphate glycosaminoglycan. J Cell Sci. 2002; 115 (23): 4495-503. doi: 10.1242/jcs.00114.

Emeigh Hart SG. Assessment of renal injury in vivo. J Pharmacol Toxicol Methods. 2005; 52 (1): 30-45. doi: 10.1016/j.vascn.2005.04.006.

Asanuma H, Vanderbrink BA, Campbell MT, et al. Arterially delivered mesenchymal stem cells prevent obstruction-induced renal fibrosis. J Surg Res. 2011; 168(1): 51-9. doi:10.1016/j.jss. 2010.06.022.

Owoade AO, Adetulu A, Olorunnisola OS. Hematological and biochemical changes in blood, liver and kidney tissues under the effect of tramadol treatment. J Alcohol Drug Depend. 2019; 7 (5): 1-7.

Tucci Junior S, Molina CAF, Cassini MF, Andrade MF de, Lima GJ de, Martins ACP. Chronic partial urethral obstruction in female rats: description of an experimental model and initial results. Acta Cirurgica Brasileira [Internet]. 2011; 26 Suppl. 2:S111-4. Available from: http://www.scielo. br/pdf/acb/v26s2/a20v26s2.pdf

Good DW, Thampi G. Neurotrophin-3 inhibits HCO3- absorption via a cAMP - dependent pathway in renal thick ascending limb. Am J Physiol Cell Physiol. 2001; 281 (6): 1804-11. doi: 10.1152/ajpcell.2001.281.6.C1804.

Lin S, Lian D, Liu W, et al. Daily therapy with a slow-releasing H2S donor GYY4137 enables early functional recovery and ameliorates renal injury associated with urinary obstruction. Nitric Oxide. 2018; 76: 16-28. doi: 10.1016/j.niox.2018.03.002.

Kaid F, Alabsi AM, Alafifi N, et al. Histological, biochemical, and hematological effects of Goniothalamin on selective internal organs of male Sprague-Dawley rats. Journal of Toxicology [Internet]. 2019 [cited 2020 Feb 17]. Available from: https://doi.org/ 10.1155/2019/6493286.

Speakman MJ, Cheng X. Management of the complications of BPH/BOO. Indian J Urol [Internet]. 2014 [cited 2020 Feb 17]; 30:208-13. Available from: http://www.indianjurol. com/ text.asp?2014/30/2/208/127856.

Zhang ZH, He JQ, Qin WW, Zhao YY, Tan NH. Biomarkers of obstructive nephropathy using a metabolomics approach in rat. Chem Biol Interact. 2018; 296: 229-39, doi: https://doi.org/ 10.1016/j.cbi.2018.10.004.

Ronco C, editor. Contributions to Nephrology. Basel (Switzerland): Karger; 2011. 372p. (Herrera GA, editor. Experimental models for renal diseases: pathogenesis and diagnosis; vol. 169).

Orth SR, Ritz E, Suter-Crazzolara C. Glial cell line-derived neurotrophic factor (GDNF) is expressed in the human kidney and is a growth factor for human mesangial cells. Nephrol Dial Transplant. 2000; 15(5): 589-95. doi: 10.1093/ndt/15.5.589.

Published

2021-09-30

How to Cite

Globa, V., Samburg, Y., Bozhok, G., & Legach, E. (2021). Biochemical blood parameters of rats with infravesical obstruction with the introduction of biologically active compositions containing neurotrophic factors. EMERGENCY MEDICINE, 16(4), 115–120. https://doi.org/10.22141/2224-0586.16.4.2020.207940

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Original Researches